ANIMAL FACILITIES (Under Construction)



Animal care and use program and facilities - Judith Daviau, DVM, TJU.
TJU has a centralized animal care and use program, which supports its research and education mission. The animal research facilities and program are accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International (AAALAC-I), have a current assurance #A3085-01 in compliance with the Public Health Service Policy and are registered as a research facility #23-R-0011 with the U.S. Department of Agriculture under provisions of the Animal Welfare Act. The animal research facilities are located on the TJU campus in 3 buildings for a total of 39,491 sft of animal research support and housing space. The facilities house a wide variety of laboratory animal species and have support areas for specialized functions including a transgenic rodent barrier facility, microinjection laboratory, Biosafety level 3 suites, experimental surgery facilities, and other diagnostic and support space.
Animal facilities are located in the center of Jefferson Alumni Hall on floors 2 through 6. These are multipurpose facilities that house a variety of animal species. The third floor contains a Biosafety Level 3 suite and also a non-human primate facility. A portion of the fourth floor contains experimental surgery suites and support areas. The fifth floor is a rodent barrier facility. 2 elevators interconnect the floors and key card security access is required. There is also a modern zebra fish aquaria laboratory on the fifth floor outside the main animal facility. The facilities are a centralized university resource and managed by the Office of Animal Resources. The director, Judith Daviau, DVM is a Diplomate of the American College of Laboratory Animal Medicine (ACLAM). A clinical veterinarian, business manager, facility manager, 4 supervisors, 4 veterinary technicians and 33 animal caretakers assist her. The Institutional Animal Care and Use Committee (IACUC) is actively involved in the program.



Laboratory animals - Arthur Buchberg, PhD, KCC, TJU.
The primary purpose of the Laboratory Animal Shared Resource is to provide the resources and environment with which animals models of cancer can be created and analyzed for the KCC. This shared resource provides housing and high quality maintenance and care for laboratory animals. The facility also provides technical expertise in small animal surgery, administration of chemical and radiological reagents. A critical component of the facility is the maintenance of a high quality barrier facility to generate a specific pathogen free environment that ensures the accurate interpretation of experimental results. The in-colony health surveillance program ensures that fidelity of the facility. The Animal Shared Resource has maintained full AAALAC accreditation since 1977. The facility is operated as a partial barrier system in which all animals are housed in sterile microisolator cages. The facility is composed of 29 animal rooms, along with the requisite cage and food storage area, a dedicated necropsy room, and a tunnel cage washer. The shared resource has consistently maintained the highest level of expertise and proficiency greatly enhancing the work of the cancer center members. The shared resource has a total capacity of ~10,600 rodent cages, a recent census indicates that the facility is operating at ~80% capacity. There is a strong need to increase capacity to enable full utilization of resources and plans are under development to address these needs.



Transgenic knock-out mice - Carlisle Landel, PhD, KCC, TJU.
The ability to produce animal models of disease with specifically engineered genetic alterations is an extremely powerful tool in cancer research. The availability of these transgenic technologies is a cornerstone of modern biological research. The KCC Transgenic Facility was established in 1992 to provide services in the production of transgenic mice by pronuclear microinjection. Over time, this service was expanded to include the production of chimeric animals via the injection of embryonic stem cells carrying targeted mutations into host blastocysts.
The microinjection suite contains all the necessary items for embryo manipulation and mouse surgery. It is equipped with 2 Leica inverted microscopes with DIC optics and Leitz micromanipulators, a Sutter P-27 pipette puller, a De Fonbrune microforge, a Sutter BV-10 micropipette beveller, dissecting microscopes for embryo manipulation and embryo transfer surgery, and incubators for embryo culture. The cryopreservation suite is the site of germplasm collection, cryopreservation and recovery, and additionally functions as the location for performing assisted reproductive techniques and producing and culturing chimeric embryos produced by aggregation. It contains 2 CBS liquid nitrogen storage tanks with a capacity of 15,000 samples, each and fitted with an automatic filling system to maintain liquid nitrogen levels, dissecting microscopes for germplasm recovery and manipulation and for embryo transfer surgery, an FTS Biocool IV controlled rate freezer, a KMINC-1000 benchtop IVF incubator, and a tissue culture incubator for embryo culture. It also contains a BLS CF-150 cell fusion apparatus for producing tetraploid embryos used in making fusion chimeras.



Office of Laboratory Animal Medicine - Frank Warren, UD.
The UD Office of Laboratory Animal Medicine (OLAM) presently maintains an approximately 11,000 square foot animal housing and research facility. OLAM has a staff of 8 full time employees including 2 administrative staff members, 4 animal health technicians, a certified and licensed laboratory animal health technician, and a licensed attending veterinarian. The animal facility includes a modern surgical suite, 2 rodent surgical rooms, a cryo-storage room, a full-function procedure laboratory, an x-ray facility, and a diagnostic laboratory. The facility provides housing for mice, rats, rabbits in all of the 20 available animal housing rooms. Additionally, the UD animal-housing facilities include a room for the housing of immune-compromised animals, an off-site quarantine room for non-approved vendor animals, and ventilated mouse housing racks. Facilities accommodate the aseptic or conventional housing for mice as well as isolation capacity for work with pathogens. The McKinly Laboratory houses the main UD laboratory animal facility and OLAM. Additional satellite facilities for psychobiological research are located in Wolf Hall. The UD animal care program is PHS approved (#50-R-0006) and USDA registered (#A3773-01).



Life Science Center - Paul Fawcett, PhD, Nemours/AIDHC.
The Life Science Center (LSC) is a core facility designed for conducting biomedical research using animal models of human disease. In spring of 2008 a roughly $360,000 renovation to the LSC (funded by the COBRE grant) was completed that will facilitate translational research programs by investigators at Nemours. The AAALAC accredited facility is led by Paul T. Fawcett, Ph.D. with support from Gwen Talham, DVM, Attending Veterinarian, Patty Wheatley, RLAT, Manage, Anne Hesek, RLAT, Surgical Services Specialist. This facility includes 10 holding rooms, 3 surgical suites and 4 procedure rooms.