STRUCTURAL BIOLOGY & CRYSTALLOGRAPHY (Under Construction)|
X-Ray crystallography - John Pascal, PhD, KCC, TJU.
The X-ray Crystallography Shared Resource (XCSR) provides TJU investigators the ability to obtain structural information directly relevant to their research interests. This includes the determination of de novo structures, structural consequences of point mutations, co-crystallization with small molecules including inhibitors and potential therapeutics, and multi-component systems including proteins, DNA and/or RNA. Although structure determination is a highly specialized, labor-intensive endeavor, the information obtained from these investigations addresses seemingly disparate biochemical data and invariably improves future research efforts.
The XCSR is located in a dedicated 1,200 sft laboratory located on the 8th floor of the Bluemle Life Sciences Building. The facility houses 2 X-ray systems: a Rigaku RU200 generator with an R-AXIS II detector with MSC Blue Confocal Optics on the right port and a state of the art RAXIS IV++ detector with MSC Blue Confocal Optics on the left port, and a Oxford Diffraction UltraPX Ultra diffractometer with a 2.2 kW sealed tube X-ray source, a 4 circle diffractometer, a Onyx CCD camera and a Cryojet. In addition, the facility houses a Matrix Technologies HydraII plus 1 crystallization robot capable of 96 and 384 well trials and dispensing volumes as low as 200 nL. Incubators are multiple temperatures, and have microscopes with photographic capabilities and crystal manipulation tools. The facility has several PCs running Linux as well as a Mac dual G5. Each computer contains the relevant software for all aspects of structure determination.
Macromolecular X-ray crystallography - Brian Bahnson, PhD, Chemistry/Biochemistry, UD.
The X-ray crystallography facility offers state-of-the-art instrumentation to solve structures of biological macromolecules from single crystals, and is open to researchers and collaborators. The protein crystallography core is funded by a NIH COBRE (Center of Biomedical Research Excellence) grant from NIH. Dr. Brian Bahnson, Core Director, works closely with multidisciplinary research groups to make optimal use of the macromolecular crystallography facilities for preliminary studies of specific research projects. Outside industry users will be accommodated on a fee-for-service basis when scheduling permits. Instruments include: Rigaku/MSC RU-H3R rotating anode X-ray generator offering a high brilliance 300 micron X-ray beam. The generator located in 314 Drake Hall is equipped with Osmic optics, which gives the beam a more tightly focused and monochromatic beam. Rigaku/MSC R-axis IV image plate area detector is located in 314 Drake Hall. The detector collects images that are 3,000 square pixels with a 100-micron pixel resolution. Data to a resolution of 1.4 Å has been collected on this X-ray system. X-stream Cryo-System for Data Collection at -180 °C: Data are typically collected under cryo conditions using this self-contained X-stream cryo-system that includes an air compressor, gas separation filter bank, He-refrigerated cryo system, and nozzle.
Structural biology - Steve Bai, PhD, Chemistry/Biochemistry, UD.
The NMR facility offers a suite of spectrometers in Brown Laboratory, and is open to researchers and collaborators. Instruments are available for liquid-state and solid state NMR spectroscopy. In addition, the facility provides spectrometer training and sample services to campus researchers, who may request instrument time or training. Instruments include: The Bruker AMX360 spectrometer is equipped with a 5-mm QNP probe and a 5-mm broadband probe. Variable temperature ranges from -70 °C to +150 °C. The Bruker AV400 spectrometer is equipped with an auto-sampler, which handles 60 samples. Remote access capability will facilitate use by other institutions of higher education in the region. The Bruker DRX-400 spectrometer is equipped with a 5-mm QNP probe and a 5-mm broadband probe. Variable temperature ranges from -70 °C to +150 °C. The Bruker AV600 spectrometer is equipped with a 5-mm inverse triple resonance CryoProbe, a conventional 5-mm inverse triple resonance probe (ATM PTXI probe), and a 5-mm broadband probe. A Dell Precision workstation (Linux Redhat 7.5 and Bruker xwinnmr 3.5) for off-line data processes