TISSUE PROCUREMENT & HISTOLOGY (Under Construction)



Translational research - Mark Curtis, MD, PhD, Hallgeir Rui, MD, PhD, TJU.
Since the inception of the Translational Research Shared Resource (TRSR) its mission has been to provide tissue processing, slide preparation, histochemical staining and immunohistochemical staining for human neoplastic tissue, and experimental animal model tissue being utilized by KCC researchers. This shared resource has also provided procurement, banking, and distribution of human neoplastic tissue in order to facilitate translational research. The recently instituted caBIG clinical database provides pathologic and clinical information corresponding to the tissue specimens present in the resource tumor/tissue bank. In addition, the TRSS provides tissue microarrays using standard and CEMA technology with paraffin archive material from the resource tumor/tissue bank. Laser microdissection of distinct cell and tissue types for molecular analysis can be performed on the resource's Laser Microdissection system. For all of the capabilities offered by the TRSS, staff is available to provide technical support and pathology expertise to KCC investigators. In 2006, 29 principal investigators from various programs of KCC benefited from this shared resource.



Tissue procurement - Brian Little, MD, PhD, CCHS.
The Tissue Procurement Center (TPC) was developed at the HFGCC in 2004, established with the goal of banking tissue appropriate for DNA and RNA extraction, tissue arrays, laser-capture microdissection, and immunohistochemistry. A total of just over 1000 tissue specimens have been collected to date, including 20 breast, 436 colon, 24 esophagus, 4 kidney, 4 larynx, 40 liver, 396 lung, 16 parotid, and 96 rectal. The TPC was 1 of the reasons for the NCI Cancer Genome Atlas Project award to the HFGCC in October 2008, which will bring additional jobs and national exposure to CCHS and HFGCC. A similar TPC also exists at TJU and will be a tremendous resource for scientists in the Delaware Center for Cancer Biology (DCCB) for innovative cancer research. Both TJU and the HFGCC are part of the National Cancer Institute caBIG, utilizing caTissue for data collection. At the NCI Community Cancer Research (NCCCR) efforts are ongoing to establish a Nemours Tissue Procurement Laboratory (NTPL). This facility will bank childhood tumor tissues and blood samples collected from 3 Nemours sites in Florida and the 1 in Wilmington, DE. As described for the HFGCC TPC, NTPL should enhance research productivity, collaboration, and funding for childhood cancer research in Delaware. Moreover, biotechnology and biomedical companies are interested in acquiring specimens for cancer research, which will serve as a nidus for collaboration with researchers in the DCCB.



Immunohistochemistry - Bruce Boman, MD, PhD, CCHS.
Establishment of an immunohistochemistry facility at the CTCR is also planned in conjunction with opening of the new HFGCC in 2009. Plans include obtaining a Ventana Benchmark XT Autostainer for this facility. This system will process immunohistochemistry, in situ hybridization, fluorescence in-situ hybridization, and fluorescein isothiocyanate tests independently or simultaneously. Complete automated processing is possible, with a throughput of 60 slides in an 8-hour period.



Histotechnology - Jeffery Twiss, MD, PhD, Nemours/AIDHC.
The Histotechnology Core is a full service histology facility providing routine and special histology technique. Services include: immunocytochemistry, immunofluorescence and immunohistochemistry; in situ hybridization, in-situ-PCR and TUNEL; H&E, special staining and basic cytology; Immuno Gold, Immuno Silver and BrdU; laser capture microdissection and immunodiagnostics. The core laboratory has been in operation since 1986, serving the hospital's research and clinical staff. It is staffed by 4 ASCP certified histology technicians and directed by Carol Barone, a board certified manager with 30 years experience.